16SFWJ_RSQ_0001

Construct

RMDB ID

16SFWJ_RSQ_0001

Name

16S rRNA Four-Way Junction

Sequence (110 nt)

GGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAAACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCC

Structure

(((..((...(((((((.((((..((.((((((....)))))).))...)))).....(((....))).....((((((.((....)))))))).)))))))..)).)))

Offset

125

# of constructs

196

# of data points

17160

Last modified

04/09/17

Version

4

Owner

Siqi Tian

Comments

RNA was heat to 90 C for 2 min and cool on ice for 2 min, then incubated at 37 C for 20 min at pH 8.0 with 10 mM MgCl2 to aid folding.
Additional sequences at 5' and 3' end not shown; two different flanking sequences were used and averaged.
Data are normalized based on GAGUA pentaloops in flanking sequences (not shown).
SHAPE normalized so that all 5 loop residues give mean reactivity of 2.0.

Annotation

• modifier: 1M7
• processing: backgroundSubtraction, overmodificationCorrection
• temperature: 24C
• chemical: Na-HEPES:50mM(pH8.0), MgCl2:10mM