ADD140_1M7_0012

Construct

RMDB ID

ADD140_1M7_0012

Name

add Riboswitch 13-140, V. vulnificus

Sequence (128 nt)

CGCUUCAUAUAAUCCUAAUGAUAUCGUUUGGGAGUUUCUACCAAGAGCCUUAAACUCUUGAUUAUGAAGUCUGUCGCUUUAUCCGAAAUUUUAUAAAGAGAAGACUCAUGAAUUACUUUGACCUGCCG

Structure

..(((((((...((((((.........))))))........((((((.......))))))..))))))).......((((((..........))))))..............................

Offset

12

# of constructs

130

# of data points

16512

Last modified

07/28/24

Version

1

Owner

Siqi Tian

Comments

Based on a mutant background, where "WT" is G37C of natural sequence.
RNA was heat to 90 C for 2 min and cool on ice for 2 min, then incubated at 37 C for 20 min at pH 8.0 with 10 mM MgCl2 and 5 mM adenine to aid folding.
Additional sequences at 5' and 3' end not shown.
Data are normalized based on GAGUA pentaloops in flanking sequences (not shown).

Annotation

• modifier: 1M7
• processing: backgroundSubtraction, overmodificationCorrection
• temperature: 24C
• chemical: Na-HEPES:50mM(pH8.0), MgCl2:10mM, adenine:5mM