ADD140_DCP_0002

Construct

RMDB ID

ADD140_DCP_0002

Name

add Riboswitch 13-140, V. vulnificus

Sequence (128 nt)

CGCUUCAUAUAAUCCUAAUGAUAUGGUUUGGGAGUUUCUACCAAGAGCCUUAAACUCUUGAUUAUGAAGUCUGUCGCUUUAUCCGAAAUUUUAUAAAGAGAAGACUCAUGAAUUACUUUGACCUGCCG

Structure

..(((((((...((((((.........))))))........((((((.......))))))..))))))).......((((((..........))))))..............................

Offset

12

# of constructs

101

# of data points

3840

Last modified

07/28/24

Version

1

Owner

Siqi Tian

Comments

Data for WT and mutant backgrounds Lock-P1, Lock-P2, Lock-P4A, Lock-P4B, and Lock-P1B are included.
RNA was heat to 90 C for 2 min and cool on ice for 2 min, then incubated at 37 C for 20 min at pH 8.0 with 10 mM MgCl2 to aid folding.
Additional sequences at 5' and 3' end not shown.
Data are normalized based on GAGUA pentaloops in flanking sequences (not shown).
SHAPE and Terbium normalized so that all 5 loop residues give mean reactivity of 1.0.
DMS normalized so that 2 A residues in loop give mean reactivity of 1.0.
Glyoxal normalized so that middle G residue in loop give mean reactivity of 1.0.
UV302 normalized so that 2 C residues in linker give mean reactivity of 1.0.

Annotation

• processing: backgroundSubtraction, overmodificationCorrection
• temperature: 24C
• chemical: Na-HEPES:50mM(pH8.0), MgCl2:10mM, adenine:5mM