ADD140_LCK_0003

Construct

RMDB ID

ADD140_LCK_0003

Name

add Riboswitch 13-140, V. vulnificus

Sequence (128 nt)

CGCUUCAUAUAAUCCUAAUGAUAUGGUUUGGGAGUUUCUACCAAGAGCCUUAAACUCUUGAUUAGCAAGUCUGUCGCUUUAUCCGAAAUUUUAUAAAGAGAAGACUGCUGAAUUACUUUGACCUGCCG

Structure

..(((((((...((((((.........))))))........((((((.......))))))..))))))).......((((((..........))))))..............................

Offset

12

# of constructs

86

# of data points

8704

Last modified

07/28/24

Version

1

Owner

Siqi Tian

Comments

Based on a mutant background, where "WT" is Lock-P4A [U77A;G78C;C119G;A120U] of natural sequence.
RNA was heat to 90 C for 2 min and cool on ice for 2 min, then incubated at 37 C for 20 min at pH 8.0 with 10 mM MgCl2 to aid folding.
Additional sequences at 5' and 3' end not shown.
Data are normalized based on GAGUA pentaloops in flanking sequences (not shown).

Annotation

• modifier: 1M7
• processing: backgroundSubtraction, overmodificationCorrection
• temperature: 24C
• chemical: Na-HEPES:50mM(pH8.0), MgCl2:10mM