ADD140_RSQ_0001

add Riboswitch 13-140, V. vulnificus

General
Reactivity heatmap for ADD140_RSQ_0001
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Construct

RMDB ID
ADD140_RSQ_0001
Name
add Riboswitch 13-140, V. vulnificus
Sequence (128 nt)
CGCUUCAUAUAAUCCUAAUGAUAUGGUUUGGGAGUUUCUACCAAGAGCCUUAAACUCUUGAUUAUGAAGUCUGUCGCUUUAUCCGAAAUUUUAUAAAGAGAAGACUCAUGAAUUACUUUGACCUGCCG
Structure
..(((((((...((((((.........))))))........((((((.......))))))..))))))).......((((((..........))))))..............................
Offset
12
# of constructs
476
# of data points
48640
Last modified
07/28/24
Version
1
Owner
Siqi Tian

Comments

RNA was heat to 90 C for 2 min and cool on ice for 2 min, then incubated at 37 C for 20 min at pH 8.0 with 10 mM MgCl2 to aid folding.
Additional sequences at 5' and 3' end not shown.
Data are normalized based on GAGUA pentaloops in flanking sequences (not shown).

Annotation

  • modifier: 1M7
  • processing: backgroundSubtraction, overmodificationCorrection
  • temperature: 24C
  • chemical: Na-HEPES:50mM(pH8.0), MgCl2:10mM

Citation

Tian S, Kladwang W, Das R Allosteric mechanism of the V. vulnificus adenine riboswitch resolved by four-dimensional chemical mapping. eLife (2018) . doi:10.7554/eLife.29602 . PMID:29446752

Notes

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