NEIL1_DMS_0009

Construct

RMDB ID

NEIL1_DMS_0009

Name

NEIL1 dsRNA library

Sequence (77 nt)

AGCCUGCCCUCUGAUCUCUGCCUGUUCCUCUGUCCCACAGGGGGCAAAGGCUACGGGCAGAGAGCGGGGAGGAGGAC

Structure

..(((.(((((((.(((((((((((.(((.((((((....)))))).)))..))))))))))).))))).)))))..

Offset

0

# of constructs

1

# of data points

77

Last modified

08/02/24

Version

1

Owner

Xin Liu

Comments

RNA was heat to 95 C for 2 min and cool on ice for 1-2 min, then incubated at 37 C for 30 min at pH 7.0 (Sodium Cacodylate) with 10 mM MgCl2 to aid folding.
Additional sequences at 5' and 3' end not shown.

Annotation

• modifier: DMS
• temperature: 37C
• chemical: Sodium Cacodylate:300mM (pH7.0), MgCl2:10mM