OK45LIB_2A3_0000

OpenKnot4_OpenKnot5_FixBB

General

Download .rdat

Construct

RMDB ID: OK45LIB_2A3_0000
Name: OpenKnot4_OpenKnot5_FixBB
Sequence (177 nt): XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Structure: .................................................................................................................................................................................
Offset: 0
# of constructs: 9143
# of data points: 914200
Last modified: 02/18/26
Version: 1
Owner: Jonathan Romano

from data: NovaSeq_2024-07-30_WK_PK50_DO-all/DO-all
merge 4 replicates
Mutational profiling

modifier: 2A3
processing: RNAFramework-v2.8.4, spread_deletions, backgroundSubtraction, normalization:value:0.0205, UBR-v1.4.1
reverse_transcriptase: SSII_Mn
temperature: 24C
chemical: MgCl2:10mM, KOAc:150mM, bicine:150mM(pH8.5)

Wipapat Kladwang, Hamish Blair, Rhiju Das, Jill Townley, Jonathan Romano, Eterna participants .