OK7ALIB_2A3_0000

Construct

RMDB ID

OK7ALIB_2A3_0000

Name

OpenKnot7a

Sequence (177 nt)

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Structure

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Offset

0

# of constructs

7808

# of data points

780700

Last modified

02/18/26

Version

1

Owner

Jonathan Romano

Comments

from data: NovaSeq_2025-01-14_WK_OK7a_RNN
merge 2 dialout subpools
Mutational profiling
merge all DMS subpools
Replaced structures for some P06 (Grapevine leafroll PKB271) sequences which were designed against a different structure

Annotation

• modifier: 2A3
• processing: RNAFramework-v2.8.4, spread_deletions, backgroundSubtraction, normalization:value:0.0659, UBR-v1.6.0
• reverse_transcriptase: SSII_Mn
• temperature: 24C
• chemical: MgCl2:10mM, KOAc:150mM, bicine:150mM(pH8.5)