OK7BLIB_2A3_0000

Construct

RMDB ID

OK7BLIB_2A3_0000

Name

OpenKnot7b

Sequence (307 nt)

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Structure

...................................................................................................................................................................................................................................................................................................................

Offset

0

# of constructs

4933

# of data points

1183680

Last modified

05/11/26

Version

2

Owner

Jonathan Romano

Comments

from data: Ultima_2025-01-19_OK7b and Ultima_2025-02-19_OK7b_II
merge 2 dialout subpools, repeated twice
Mutational profiling
merge all DMS subpools

Annotation

• modifier: 2A3
• processing: RNAFramework-v2.8.4, spread_deletions, backgroundSubtraction, normalization:value:0.0245, UBR-v1.6.0
• reverse_transcriptase: SSII_Mn
• temperature: 24C
• chemical: MgCl2:10mM, KOAc:150mM, bicine:150mM(pH8.5)