P90LIB_DMS_0001

Construct

RMDB ID

P90LIB_DMS_0001

Name

OpenKnotPilot_PK90

Sequence (155 nt)

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Structure

...........................................................................................................................................................

Offset

0

# of constructs

2174

# of data points

236857

Last modified

02/22/24

Version

1

Owner

Jonathan Romano

Comments

from data: NovaSeq_2023-08-02_RH_VW_MutMap/PK90_MutMap_REPROCESS2
Mutational profiling
Library ordered from CustomArray

Annotation

• modifier: DMS
• processing: RNAFramework-v2.8.4, spread_deletions, backgroundSubtraction, normalization:value:0.0249, UBR-v1.2.2
• reverse_transcriptase: Marathon
• temperature: 24C
• chemical: MgCl2:10mM, KOAc:150mM, bicine:150mM(pH8.5)