PSL2IAV_1M7_0001
Packaging Stem-Loop 2, Influenza A virus
General
Construct
- RMDB ID
PSL2IAV_1M7_0001- Name
- Packaging Stem-Loop 2, Influenza A virus
- Sequence (59 nt)
- CGACACUAAUUGAUGGCCAUCCGAAUUCUUUUGGUCGCUGUCUGGCUGUCAGUAAGUAU
- Structure
- —
- Offset
- 29
- # of constructs
- 61
- # of data points
- 3540
- Last modified
- 07/28/24
- Version
- 1
- Owner
- Rhiju Das
Comments
RNA was heat to 90 C for 2 min and cool on ice for 2 min, then incubated at 37 C for 20 min at pH 8.0 with 10 mM MgCl2 to aid folding. Additional sequences at 5' and 3' end not shown. Data are normalized based on GAGUA pentaloops in flanking sequences (not shown). Secondary structure is for reference only.
Annotation
- modifier: SHAPE
- temperature: 24C
- chemical: Na-HEPES:50mM(pH8.0), MgCl2:10mM
Citation
Hagey RJ, Elazar M, Pham EA, Tian S, Ben-Avi L, Bernardin-Souibgui C, Yee MF, Moreira FR, Rabinovitch MV, Meganck RM, Fram B, Beck A, Gibson SA, Lam G, Devera J, Kladwang W, Nguyen K, Xiong A, Schaffert S, Avisar T, Liu P, Rustagi A, Fichtenbaum CJ, Pang PS, Khatri P, Tseng CT, Taubenberger JK, Blish CA, Hurst BL, Sheahan TP, Das R, Glenn JS Programmable antivirals targeting critical conserved viral RNA secondary structures from influenza A virus and SARS-CoV-2. Nature medicine (2022) . doi:10.1038/s41591-022-01908-x . PMID:35982307