PSL2IAV_RSQ_0001
Packaging Stem-Loop 2, Influenza A virus
General
Construct
- RMDB ID
PSL2IAV_RSQ_0001- Name
- Packaging Stem-Loop 2, Influenza A virus
- Sequence (59 nt)
- CGACACUAAUUGAUGGCCAUCCGAAUUCUUUUGGUCGCUGUCUGGCUGUCAGUAAGUAU
- Structure
- ....(((.(((((((((((..(((..........))).....))))))))))).)))..
- Offset
- 29
- # of constructs
- 171
- # of data points
- 8024
- Last modified
- 12/09/17
- Version
- 1
- Owner
- Menashe Elazar
Comments
RNA was heat to 90 C for 2 min and cool on ice for 2 min, then incubated at 37 C for 20 min at pH 8.0 with 10 mM MgCl2 to aid folding. Additional sequences at 5' and 3' end not shown. Data are normalized based on GAGUA pentaloops in flanking sequences (not shown). Secondary structure is for reference only.
Annotation
- modifier: 1M7
- processing: backgroundSubtraction, overmodificationCorrection
- temperature: 24C
- chemical: Na-HEPES:50mM(pH8.0), MgCl2:10mM
Citation
Rachel J. Hagey, Menashe Elazar, Siqi Tian, Edward A. Pham, Wipapat Kladwang, Khanh Nguyen, Anming Xiong, Philip S. Pang, Steven Schaffert, Purvesh Khatri, Jeffery K. Taubenberger, Rhiju Das, Jeffrey S. Glenn Identification and targeting of a pan-genotypic influenza A virus RNA structure that mediates packaging and disease.