RNAPZ5_RSQ_0001

Construct

RMDB ID

RNAPZ5_RSQ_0001

Name

RNA Puzzle 5

Sequence (188 nt)

GGUUGGGUUGGGAAGUAUCAUGGCUAAUCACCAUGAUGCAAUCGGGUUGAACACUUAAUUGGGUUAAAACGGUGGGGGACGAUCCCGUAACAUCCGUCCUAACGGCGACAGACUGCACGGCCCUGCCUCUUAGGUGUGUUCAAUGAACAGUCGUUCCGAAAGGAAGCAUCCGGUAUCCCAAGACAAUC

Structure

(((((..(((((..(((((((((.......)))))))))..((((.((((((((......(((((...((((((((((....))))....)).))))((....)).....[[[[[...))))).((((...))))))))))))....]]]]].((((....))))....))))...)))))..)))))

Offset

63

# of constructs

171

# of data points

25568

Last modified

07/28/24

Version

3

Owner

Siqi Tian

Comments

RNA was heat to 90 C for 2 min and cool on ice for 2 min, then incubated at 37 C for 20 min at pH 8.0 with 10 mM MgCl2 to aid folding.
Additional sequences at 5' and 3' end not shown.
Data are normalized based on GAGUA pentaloops in flanking sequences (not shown).
Secondary structure is for reference only.

Annotation

• modifier: 1M7
• processing: backgroundSubtraction, overmodificationCorrection
• temperature: 24C
• chemical: Na-HEPES:50mM(pH8.0), MgCl2:10mM