RYOSFL_MOD_0006

Construct

RMDB ID

RYOSFL_MOD_0006

Name

RYOS-Followup6-nhsu #1

Sequence (107 nt)

GGAAAUAAAUAAAUAACAAUAAAGAGAUAAGACACAAUAAAUAAAAUAAUAAUGAAUAAAUAAAUAAAGAGGAGCUUCGGUUUUUCAAAAGAAACAACAACAACAAC

Structure

....................................................................(((((((....))))))).....................

Offset

0

# of constructs

1

# of data points

1044

Last modified

07/28/24

Version

1

Owner

Hannah Wayment-Steele

Comments

Inline hydrolysis (degradation) vs. SHAPE for individual constructs probed by CE (capillary electrophoresis).
RNA was transcribed with standard nucleotides, with 5-methyl-cytidine instead of C, with pseudouridine instead of U, or with 1-methyl-pseudouridine instead of U
RNA was incubated in 50 mM CHES pH 10, 10 mM MgCl2 for 40 Hr; or treated with DMS or SHAPE reagent 1M7 at pH 8, 10 mM MgCl2.
Data are normalized so that numbers reflect modification rates *per hour*.
Note: Taken in Nov. 2020 to followup on observations that PSU stabilize against degradation, 110620_RYOS-I-Followup-ModifiedRNA.

Annotation

• processing: backgroundSubtraction, overmodificationCorrection
• temperature: 24C