SRPU21C_BZCN_0001

Construct

RMDB ID

SRPU21C_BZCN_0001

Name

SRP RNA U21C, E. coli

Sequence (131 nt)

AUCGGGGGCUCUGUUGGUUCCCCCGCAACGCUACUCUGUUUACCAGGUCAGGUCCGGAAGGAAGCAGCCAAGGCAGAUGACGCGUGUGCCGGGAUGUAGCUGGCAGGGCCCCCACCCGGGUCGGCAUGGCA

Structure

...................................................................................................................................

Offset

0

# of constructs

113

# of data points

8456

Last modified

02/01/21

Version

1

Owner

Angela Yu

Comments

REACTIVITY and READS are stored below in groups for each replicate. Reactivity is first, then positive reads, followed by unmodified reads
Reactivities are reported as rhos
rho_i = theta_i * L, where L = length of RNA sequence
See Aviran, S., Lucks, J.B., and Pachter, L. (doi:10.1109/Allerton.2011.6120379) for theta_i and beta_i reactivity definitions

Annotation

• experiment: cotranscriptional SHAPE-Seq
• modifier: BzCN
• chemical: Tris-acetate:20mM (pH8.0) chemical:MgCl2:5mM chemical:EDTA:0.1mM chemical:DTT:1mM chemical:KCl:50mM chemical:NTPs:200uM chemical:BSA:1mg/mL temperature:37C