TRP4P6_RSQ_0001

Construct

RMDB ID

TRP4P6_RSQ_0001

Name

P4-P6 domain, Tetrahymena ribozyme

Sequence (160 nt)

GGAAUUGCGGGAAAGGGGUCAACAGCCGUUCAGUACCAAGUCUCAGGGGAAACUUUGAGAUGGCCUUGCAAAGGGUAUGGUAAUAAGCUGACGGACAUGGUCCUAACCACGCAGCCAAGUCCUAAGUCAACAGAUCUUCUGUUGAUAUGGAUGCAGUUCA

Structure

.....((((((...((((((.....(((.((((.(((..(((((((((....)))))))))..((.......))....)))......)))))))....))))))..)).))))((...((((...(((((((((...)))))))))..))))...))...

Offset

101

# of constructs

101

# of data points

12800

Last modified

07/28/24

Version

2

Owner

Siqi Tian

Comments

RNA was heat to 90 C for 2 min and cool on ice for 2 min, then incubated at 37 C for 20 min at pH 8.0 with 10 mM MgCl2 to aid folding.
Additional sequences at 5' and 3' end not shown.
Data are normalized based on GAGUA pentaloops in flanking sequences (not shown).
Secondary structure is for reference only.

Annotation

• modifier: 1M7
• processing: backgroundSubtraction, overmodificationCorrection
• temperature: 24C
• chemical: Na-HEPES:50mM(pH8.0), MgCl2:10mM