ZTPRSW_BZCN_0010

Construct

RMDB ID

ZTPRSW_BZCN_0010

Name

pfl ZTP Riboswitch, C. beijerinckii, 1 mM ZMP, SSIV RT, Replicate 2

Sequence (157 nt)

AUAUUAGAUAUUAGUCAUAUGACUGACGGAAGUGGAGUUACCACAUGAAGUAUGACUAGGCAUAUUAUCUUAUAUGCCACAAAAAGCCGACCGUCUGGGCAAAAAAAGCCUGGAUUGCGUCGGCUUUUUUAUAUGGAAAAGGAGGAAGGAUCUAUGA

Structure

.............................................................................................................................................................

Offset

0

# of constructs

10

# of data points

1352

Last modified

11/04/19

Version

1

Owner

Eric Strobel

Comments

REACTIVITY and READS are stored below in groups for each replicate. Reactivity is first, then positive reads, followed by unmodified reads
Reactivities are reported as rhos
rho_i = theta_i * L, where L = length of RNA sequence
See Aviran, S., Lucks, J.B., and Pachter, L. (doi:10.1109/Allerton.2011.6120379) for theta_i and beta_i reactivity definitions
Cotranscriptionally folded C. beijerinckii pfl ZTP riboswitch with 1mM ZMP, 30 seconds of transcription, SSIV reverse transcription
Linker sequences removed

Annotation

• experiment: cotranscriptional SHAPE-Seq
• modifier: BzCN
• chemical: ZMP:1mM chemical:DMSO:2% chemical:Tris-acetate:20mM (pH8.0) chemical:MgCl2:10mM chemical:EDTA:0.1mM chemical:DTT:1mM chemical:KCl:50mM chemical:NTPs:500uM chemical:BSA:1mg/mL temperature:37C